The Sports face Brighton and Hove Albion at Lancing’s home ground, for the right to square up to Crawley Town in the fight for the trophy, which is currently held by Eastbourne.
Head of Professional Development at the Albion, Vic Bragg - himself a former Crawley Town player as well as a past England Schools manager - expects a close game saying, "We know it won't be easy but we're looking forward to it and are keen to do well. Eastbourne Borough are a good side with plenty of ex-pros but it's a chance to reach a cup final so there's plenty to play for. At our level, it's a bit of a balancing act in trying to teach the right things and also get results."
Borough boss, Tommy Widdrington takes a realistic but positive view saying, "We're a very young side but I've been pleased with our form in the last fifteen or so games and you never know in a one-off match.
"We've been a bit unlucky with one or two injuries - now cleared up - but while we have been scoring regularly, we've tended to be a bit loose at the other end. We've set out our stall to be more attacking and I can't see this game being a drab affair.
"It would be good to have another big night out at the Amex to look forward to. Once you start progressing in a competition the more a final place figures on the list. It's good to be the champions of your county. Ok, on seedings we should probably be in the last four, but it would be icing on the cake to reach the final and then retain the trophy."
Widdrington will know what to expect from Albion's talented youngsters, "I've seen them a couple of times in the last month because it's my business to know what players are about at this level."
Kick off at Culver Road is at 7.15pm to allow for the possibility of extra time, owing to a planning agreement, which prevents the use of floodlights after 10pm at the West Sussex ground.